A research paper entitled "Aberrant neuronal connectivity in the cortex drives generation of seizures in rat absence epilepsy" was recently published in Brain journal.
The research team used MoreHisto software as an automated histological quantification program to estimate cellular densities and fluorescence intensity for the wS1 cortex based on the expression of specific markers (NeuN, RECA, Hoechst, GFAP, Iba1) on horizontal brain sections.
To know more about the research done, you can check this link.